Methods in Cardiomyocyte Isolation, Culture, and Gene Transfer - adult cardiomyocytes

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adult cardiomyocytes - Cardiac muscle cell - Wikipedia


May 21, 2014 · There are some atrial cardiomyocyte cell lines, like HL-1 and AT-1 cells derived from mouse atrial tumors; however, there are no adult ventricular cardiomyocyte cell lines available for research. Primary cell cultures of adult mouse cardiomyocytes provide a powerful model for heart research at the cellular and molecular levels.Cited by: 23. Adult cardiomyocytes that are fully differentiated are generally reported to be poor candidates for non-viral gene transfer. However, greater success has been achieved with transfection of neonatal cardiomyocytes using approaches such as liposomal reagents, electroporation, and optical transfection.Cited by: 355.

A healthy adult cardiomyocyte has a cylindrical shape that is approximately 100μm long and 10-25μm in diameter. Cardiomyocyte hypertrophy occurs through sarcomerogenesis, the creation of new sarcomere units in the cell. During heart volume overload, cardiomyocytes grow through eccentric hypertrophy.MeSH: D032383. Origin of Cardiomyocytes in the Adult Heart. Annarosa Leri, Marcello Rota, Francesco S. Pasqualini, Polina Goichberg, and ; Piero Anversa; From the Departments of Anesthesia and Medicine and Division of Cardiovascular Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA.Cited by: 54.

The isolation of viable adult mouse cardiomyocytes for short-term culture alone is known to be a challenging task. This protocol provides step-by-step instructions on how to achieve viable cardiomyocytes from adult mice that can be used for both short-term as well as long-term investigations.Cited by: 2. This is a hurdle indeed, given that unlike zebrafish cardiomyocytes, a high percentage (∼ 75%) of adult mouse cardiomyocytes are binucleate (Li et al., 1996). However, even that rare cell cycle entry events occur suggests that there is opportunity for enhancing a naturally occurring process.

Apr 06, 2018 · During development, fetal cardiomyocytes rapidly divide to create heart tissue. This regenerative ability is lost in adult cardiomyocytes, which are unable to divide because they’ve already exited the cell cycle (a series of phases that a cell goes through that ultimately results in its division). With the method of isolation and cultivation of adult cardiomyocytes, a precise investigation of the behavior of these cells under specific treatments and environments is possible. This manuscript presents a protocol for successful isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC).Cited by: 4.

Adult cardiomyocytes retain their characteristic structure after isolation, although this gradually diminishes over days in culture unless the cells are electrically stimulated. In addition to the difference in size, marked differences in morphology are apparent between the two types of cardiomyocyte cultures, with rectangular adult Cited by: 116.